Food Control™ qPCR

$430.00

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Description

Food Control™ qPCR is a kit for fast and reliable detection of foodborne pathogens via real-time PCR (qPCR).

Detecting bacterial contaminations is a key aspect in food safety testing that minimizes the risk of intoxicating consumers. The increasing public awareness of food safety calls for extensive microbiological quality control programs throughout the food chain production. In this context, reliability, speed, and robustness of a test system to detect the presence or absence, or even the degree of contamination with pathogens, becomes increasingly important.

Food Control™ qPCR is a diagnostic system for easy determination of contamination degree in agricultural or food industry via real-time PCR.
The kit enables detection of down to 10 DNA genome copies/assay of the targeted species, in about 150 minutes. Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth medium. DNA extracts can be conveniently obtained with the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user-based method.

Type of PCR

Real-time PCR. TaqMan® assay based on FAM™ and HEX™ labeled probes.

Recommended Use

Applicable in research and industry for detection of pathogenic contamination in food or agricultural products. For research use only! Do not use for clinical diagnostic or testing of patient samples.

The kit is available for the detection of the following microorganisms via semi-quantitative real-time PCR:

Microorganism Target
Salmonella enterica
invasion protein A (invA) gen
Yersinia enterocolitica heat stable enterotoxin A gen
Shigella spp. invasion plasmid antigen (ipaH6) gen
Campylobacter spp. acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase (lpxA) gen
Clostridium perfringens phospholipase C alpha toxin (plc) gen
Shigella dysenteriae, Escherichia coli (EHEC) Shiga Toxin 1 (stx1) gen
Shigella dysenteriae, Escherichia coli (EHEC) Shiga Toxin 2 (stx2) gen
Escherichia coli O157 wbdR gen
Escherichia coli O104 wckD gen
Listeria spp. invasion associated protein p60 (iap) gen
Listeria monocytogenes listeriolysin O (hly) gen
Salmonella spp. spacer-region between 16S and 23S RNA genes
Kit Components

Lyophilized Species qPCR Mix (qPCR Mix for each species listed above): Primers / Probes / Nucleotides / Polymerase
Rehydration Buffer
Lyophilized Positive Control DNA
Lyophilized Internal Control DNA
PCR Grade Water

Package Sizes and Ordering Information

Available kits (25 reactions each) for the species listed below:

  • Cat. No. 11-02-01-025 Salmonella enterica
  • Cat. No. 11-02-02-025 Yersinia enterocolitica
  • Cat. No. 11-02-03-025 Shigella spp.
  • Cat. No. 11-02-04-025 Campylobacter spp.
  • Cat. No. 11-02-05-025 Clostridium perfringens
  • Cat. No. 11-02-06-025 Shiga Toxin 1
  • Cat. No. 11-02-07-025 Shiga Toxin 2
  • Cat. No. 11-02-08-025 Escherichia coli O157
  • Cat. No. 11-02-09-025 Escherichia coli O104
  • Cat. No. 11-02-10-025 Listeria spp.
  • Cat. No. 11-02-11-025 Listeria monocytogenes
  • Cat. No. 11-02-12-025 Salmonella spp.
Required Consumables

PCR reaction tubes, 1.5 ml reaction tubes, filter tips

Optional: DNA can be extracted from enrichment broth using the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user-based method.

Required Lab Devices

qPCR cycler with FAM™ and HEX™ filter
Pipettes
Benchtop centrifuge for 1.5 ml reaction tubes

Shelf Life and Storage

Components can be stored at +2 to +8 °C for at least 12 months. After rehydration the reagents must be stored at ≤ -18 °C.

Compliance

No, certification in progress.

Additional information

Species

Salmonella enterica, Yersinia enterocolitica, Shigella spp., Campylobacter spp., Clostridium perfringens, Shiga Toxin 1, Shiga Toxin 2, Escherichia coli O157, Escherichia coli O104, Listeria spp., Listeria monocytogenes, Salmonella spp.

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