SwabUp™ Lab Monitoring Plus kit is a contamination-free system for sampling, extraction, and PCR testing of DNA collected from various lab surfaces. The kit is designed to support environmental monitoring of DNA contaminations in molecular biology laboratories.
Originating from aerosols, splashing or dripping during sample handling, pipetting or centrifugation, small amounts of contaminant DNA are very hard to remove, easily amplified and can lead to cross-contaminations between samples. Contamination monitoring procedures are strongly recommended, when not required, by several quality assurance guidelines (e.g. for forensic testing facilities). Undertaking periodic testing of DNA contaminations is essential to ensure reliable data, to assess the performance of cleaning procedures and to prevent future occurrence.
SwabUp™ Lab Monitoring Plus kit contains a sample collection and a DNA extraction system, optimized for the efficient detection of minimal amounts of contaminant DNA, and a contamination-free PCR system. SwabUp™ Lab Monitoring Plus kit introduces the contamination-free SwabUp™ DNAmp Mix, a universal PCR master mix for both conventional and qPCR.
SwabUp™ DNAmp Mix contains all PCR core components. Only reaction-specific primers, probes (only required for qPCR) and collected samples are added by the user.
SwabUp™ Lab Monitoring kit is for research use only. It is not recommended for clinical and diagnostic applications or for the detection of RNA contaminations.
This kit is recommended for regular monitoring of lab work areas and detection of target or amplicon DNA contaminations, but especially for severe and recurrent DNA contaminations, to exclude the presence of contaminant DNA in the PCR reagents. To this aim, we recommend selecting the most frequently used primer pairs or those showing the highest recurrence of irregularities or unspecific results.
Swabs (individually packaged) and Collection Buffer tubes, Spin column-based DNA extraction system, SwabUp™ DNAmp Mix, Rehydration Buffer, MgCl2.
- Cat. No. 182-0010 10 applications (25 PCR reactions)
- Cat. No. 182-0050 50 applications (125 PCR reactions)
Ethanol (> 96 % abs.), 1.5 ml and PCR reaction tubes, filter tips, primers, and probes (only for qPCR).
Required Lab Devices
Microcentrifuge, heat block, pipettes, PCR or qPCR cycler, agarose gel electrophoresis system (only for conventional PCR).
Shelf Life and Storage
The DNA extraction system should be stored at room temperature, the Collection Buffer tubes must be stored at +2 – +8 °C. Swabs can be stored at +2 – +30 °C. SwabUp™ DNAmp Mix should be stored at +2 – +8 °C, and after rehydration at ≤ -18 °C.