Venor®GeM OneStep


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Venor®GeM OneStep is a kit for direct detection of mycoplasma contaminations in cell cultures, cell culture media, and other biological matrices by conventional PCR. Designed to require minimal pipetting, the kit includes all reagents required for PCR: primers, nucleotides, polymerase and the internal amplification control, which are provided in a ready-to-use lyophilized reaction mix. Upon rehydration with the included buffer, the mix is ready to be aliquoted according to the sample number into reaction tubes, where only samples or Positive Control DNA are added prior to PCR.

Type of PCR

For conventional, endpoint PCR with gel electrophoresis

Recommended Use

Applicable in research and industry for direct testing of cell cultures and biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Lyophilized Mix: Primer sets / Nucleotides / Internal Control DNA and polymerase in aliquots of 25 reactions each
Rehydration Buffer
Lyophilized Positive Control DNA
PCR Grade Water

Package Sizes
  • Cat. No. 11-8025 25 Reactions
    Cat. No. 11-8100 100 Reactions
    Cat. No. 11-8250 250 Reactions
Result Evaluation

Gel electrophoresis at endpoint of PCR

Required Consumables

Agarose gels and DNA stains
PCR reaction tubes

Required Lab Devices

Gel electrophoresis and documentation system / Regular PCR cycler
Pipetting equipment
Tube centrifuge

Shelf Life and Storage

Venor®GeM OneStep components are maintainable at +2 to +8 °C for at least 6 months. After rehydration the reagents must be stored at ≤ -18 °C.

EP 2.6.7 compliance


Additional information

package sizes

25 tests, 100 tests, 250 tests


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