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AquaScreen® qPCR Detection Kits

The AquaScreen® qPCR Detection kit series is specifically designed for the quantitative detection of common water pathogens such as Legionella, Pseudomonas, and E.coli.The AquaScreen® approach includes sample preparation using the AquaScreen® FastExtract kit and various qPCR detection kits. In contrast to time-consuming cell culture methods, the AquaScreen® approach needs less than six hours including sample preparation and qPCR to reliably detect water pathogens. In addition, our qPCR assay is superior in terms of sensitivity and specificity as the assay only detects the respective species.The AquaScreen® qPCR assay is insensitive to contamination with other bacterial genera. Also, the assay’s robustness is unsurpassed with linear detection up to 3 x 106 particles per sample.

AquaScreen® Legionella pneumophila

Features

Type of PCR

Quantitative real-time PCR (qPCR)

Description

The AquaScreen® Legionella pneumophila qPCR Detection kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Legionella pneumophila in waters samples. The used primer set is specific for a segment of the mip region of the Legionella pneumophila genome.

Scope of Application

Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components

Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes
  • Cat. No. 34-2025 25 reactions
    Cat. No. 34-2100 100 reactions
    Cat. No. 34-2250 250 reactions
Required Consumables

PCR reaction tubes
Optional: For quantification we recommend our Legionella pneumophila PCR Quantification Standard (Cat. No. 52-0101).

Required Lab Equipment

qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage

The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.

Compliance

AFNOR XP T90-471 and ISO/TS 12869:2012

AquaScreen® Legionella species

Features

Type of PCR

Quantitative real-time PCR (qPCR)

Description

The AquaScreen® Legionella species qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® Fast Extract kit for quantification of Legionella in waters samples. The supplied primer set is specific for a broad range of legionella species, but does not detect other waterborne bacteria as required by ISO/TS 12869:2012.

Scope of Application

Applicable for water testing as described in ISO/TS 12869:2012, in research and industry for QA testing of process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components

Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes
  • Cat. No. 33-2025 25 reactions
    Cat. No. 33-2100 100 reactions
    Cat. No. 33-2250 250 reactions
Required Consumables

PCR reaction tubes
Optional: For quantification we recommend our Legionella pneumophila PCR Quantification Standard (Cat. No. 52-0101).

Required Lab Equipment

qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage

The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below         -18 °C.

Compliance

AFNOR XP T90-471 and ISO/TS 12869:2012

AquaScreen® Pseudomonas aeruginosa

Features

Type of PCR

Quantitative real-time PCR (qPCR)

Description

The AquaScreen® Pseudomonas aeruginosa qPCR Detection kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Pseudomonas aeruginosa in waters samples.

Scope of Application

Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components

Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes
  • Cat. No. 34-6025 25 reactions
    Cat. No. 34-6100 100 reactions
    Cat. No. 34-6250 250 reactions
Required Consumables

PCR reaction tubes
Optional: For quantification we recommend our Pseudomonas aeruginosa PCR Quantification Standard (Cat. No. 52-0071).

Required Lab Equipment

qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage

The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below         -18 °C.

AquaScreen® Escherichia coli

Features

Type of PCR

Quantitative real-time PCR (qPCR)

Description

The AquaScreen® Escherichia coli qPCR Detection kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Escherichia coli in waters samples.

Scope of Application

Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components

Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes
  • Cat. No. 34-7025 25 reactions
    Cat. No. 34-7100 100 reactions
    Cat. No. 34-7250 250 reactions
Required Consumables

PCR reaction tubes
Optional: For quantification we recommend our E. coli PCR Quantification Standard (Cat. No. 52-0083).

Required Lab Equipment

qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage

The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below             -18 °C.

$416.00$3,339.00

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