Cat. no.: 10-0200

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Mynox® Technical Note for Mynox®

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Mynox® is a mycoplasma removal agent for treatment of contaminated cell cultures. Mycoplasma elimination is achieved in only 6 - 8 days (plus 4 passages for DNA washout).

The classic Mynox® avoids the use of typical antibiotics and instead contains surfactin, a cyclic lipopeptide. This prevents the development of resistant strains. The mode of action of Mynox® is based on a biophysical mechanism to integrate into the membrane of mycoplasmas and thereby impair their integrity

Recommended Use

Mynox® is intended for research use only. Not applicable for clinical treatment. Protocols for the direct treatment of viruses are described in the product manual.


Mynox® is a sterile, ready-to-use solution, aliquoted per tube for single use, 200 µl per vial.

Package Sizes

1 application can be used to permanently eliminate mycoplasma from one cell culture.

  • Cat. No. 10-0200 2 applications
    Cat. No. 10-0500 5 applications
    Cat. No. 10-1000 10 applications
Result Evaluation

Successful elimination of mycoplasma should be verified with a suitable mycoplasma detection system, e.g. Minerva Biolabs’s Venor®GeM Mycoplasma PCR Detection Kits.

Required Consumables

Tissue culture petri dish (60 mm Ø)
Standard cell culture plasticware

Required Lab Devices

Standard cell culture equipment (incubator, centrifuge, pipettes, etc.)

Shelf Life and Storage

Store the components at +2 °C to +8 °C until the expiry date indicated on the label.

Fig. Electron micrograph showing the effects of Mynox® when applied to contaminated cell cultures. (A) Mycoplasma contaminated cell culture before treatment. (B) With one application of Mynox® membranes of all mycoplasma are destroyed while the tissue cell membrane is unaffected. Black arrows indicate mycoplasmas, white arrows indicate parts of a tissue cell.

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