Proteinase K

Proteinase K is a proteolytic enzyme. The enzyme uses Ca²⁺ ions for structural stability. Due to the high total activity of the enzyme, lysis can be carried out in the presence of EDTA. Proteinase K is therefore excellently useful to digest contaminating proteins and improve the extraction of nucleic acids from various biological samples.

Recommended Use

The Venor®GeM Sample Preparation Kit from Minerva Biolabs is designed for highly efficient extraction of mycoplasma DNA from standard samples, like cell culture supernatants containing up to 10⁶ cells. However, other samples matrices like cell pellets, serum, or fermentation products may contain proteins at a concentration higher than 10 mg/ml. These samples may require an additional treatment with Proteinase K to increase the overall DNA yield and reach highest sensitivity.
Optional in combination with Venor®GeM Sample Preparation Kit (Minerva Biolabs) or Microsart® AMP Extraction Kit (Sartorius): Use 10 µl of the rehydrated Proteinase K to treat each sample immediately after addition of Conditioner (Venor®GeM Sample Preparation Kit) or Buffer A (Microsart® AMP Extraction). Continue as indicated in the respective manuals.

Content

Proteinase K: 1 vial, lyophilized
Rehydration Buffer: 1 vial
Dissolve the lyophilized Proteinase K in 550 µl of the included rehydration buffer.

Package Sizes

• Cat. No. 56-0002 50 extractions

Shelf Life and Storage

The lyophilized product shall be stored at +2 to +8 °C. The rehydrated stock solution must be stored at ≤ -18 °C. Avoid repeated freezing and thawing. Preparation of aliquots might be required if more than 5 freezing/thawing cycles are necessary.