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PCR Quantification Standards
  • PCR Quantification Standards
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PCR Quantification Standards

Defined DNA standards for quantitative determination of microbial organisms in PCR-based detection methods.
- Broad range of organisms available: bacteria, yeasts, fungi, and mycoplasma
- Contains a defined number of genome copies/vial (1x10⁶ or 1x10⁸)
- Suitable for standard curves, quantification, method verification, and cross-detection testing
- Non-infectious

Cat. no.: 52-0101

$540.00
Tax excluded

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Manual.pdf
Technical Notes
PCR Quantification Standards Technical Note for PCR Quantification Standards

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Product Description

Our PCR Quantification Standards contain genomic DNA extracted from defined microorganisms, at low culture passage number. The DNA starting material is analyzed with standardized methods to determine the DNA’s integrity and quantity. Only pure and intact DNA showing a > 10 kb band in an agarose gel and an OD260/280 > 1.8 is used for the manufacture of these products. The DNA is partially sequenced to confirm the species’ identity.

Recommended Use

PCR Quantification Standards contain a defined number of genome copies of a particular microorganism. Therefore, our standards are perfectly suitable to generate DNA standard curves in qPCR. In addition, standards are required for validating the specificity and testing the sensitivity of PCR assays in the context of quality control and EP 2.6.7 compliant-testing.

Content

1 vial with 1x10⁶ or 1x10⁸ genomes (lyophilized)
3 vials with Tris buffer (10 mM, pH 8.5)

Available Species and Ordering Information
  • Cat. No. 52-1988 Aspergillus brasiliensis
  • Cat. No. 52-0819 Aspergillus fumigatus
  • Cat. No. 52-0347 Bacillus subtilis
  • Cat. No. 52-1447 Bacteroides vulgatus
  • Cat. No. 52-5571 Bordetella pertussis
  • Cat. No. 52-1386 Candida albicans
  • Cat. No. 52-1226 Candida glabrata
  • Cat. No. 52-6128 Candida krusei
  • Cat. No. 52-1953 Candida tropicalis
  • Cat. No. 52-1664 Clostridium sporogenes
  • Cat. No. 52-0083 Escherichia coli
  • Cat. No. 52-0348 Kocuria rhizophila
  • Cat. No. 52-0079 Lactobacillus acidophilus
  • Cat. No. 52-0101 Legionella pneumophila
  • Cat. No. 52-0848 Penicillium chrysogenum
  • Cat. No. 52-0071 Pseudomonas aeruginosa
  • Cat. No. 52-0799 Staphylococcus aureus
  • Cat. No. 52-3317 Streptococcus bovis
Shelf Life and Storage

The components must be stored between +2 and +8 °C (Shipping at ambient temperature).